Figure 2.

Anti-laminin γ1 antibody or soluble laminin-1 specifically disrupts the endogenous laminin matrix. Adult male mice were unilaterally infused with purified laminin-1 (0.5 mg/ml), fibronectin (0.5 mg/ml), or anti-laminin γ1 antibody (0.2 mg/ml) for 7 d, brain sections were prepared and stained with anti-laminin antibody (A, B, and D), or anti-M2 muscarinic acetylcholine receptor antibody (C). Infusion of soluble laminin-1 or anti-laminin γ1 antibodies disrupted endogenous laminin in the hippocampal neuronal cell layers in the CA1 and DG regions (A and B), but did not affect the distribution of the M2 muscarinic acetylcholine receptor in the same region (C). Infusion of soluble fibronectin did not affect endogenous laminin (D). Ln, laminin-1 immunostaining; M2, M2 muscarinic acetylcholine receptor immunostaining. Bar in D, 500 μm.