Figure 3.

Plasmin digested laminin-1 can effectively disrupt the endogenous laminin matrix. (A) Purified mouse laminin-1 was digested with tPA alone or tPA plus human plasminogen at 37°C for 4 h, electrophoresed on a 10–20% reducing gradient SDS-PAGE, and stained with Coomassie Blue. The migration positions of the α chain and the βγ chains are indicated. tPA alone does not cleave laminin-1 (lane 3), but plasmin (tPA + plasminogen)-digested laminin-1 to multiple fragments (lane 4). (B and C) Wild-type mice were infused with either purified soluble laminin-1 (B) or plasmin digested laminin-1 (C), brain sections were prepared, and stained with anti-laminin-1 antibody. Bar in C, 500 μm.