Figure 2.

Myo6 overlaps with GIPC, transferrin receptor, and Rab5 on vesicles distinct from clathrin-coated pits and EEA1-positive early endosomes. (A–F) ARPE-19 cells were double labeled with antibodies directed to the tail domain of myo6 (M6; detected with a fluorescein isothiocyanate-conjugated secondary antibody) and to components of the endocytic pathway (detected with a rhodamine-conjugated secondary antibody). The top panels show an example of the whole-cell staining pattern seen for myo6 and the markers. The boxed area in the top panels is enlarged in the bottom panels. In the overlay, myo6 is shown in green, the markers are shown in red, and overlap between the two markers is yellow. Staining was for clathrin heavy chain (A) the clathrin adaptor AP-2 (B), Rab5 (C), EEA1 (D), TsfnR (E), and GIPC (F). Bars (A–F), 10 μm.