Figure 2.

Translational repression is not an aspect of the S. cerevisiae UPR. Wild-type cells were treated with the glycosylation inhibitor tunicamycin for the indicated times, followed by a 10-min pulse-label with [³⁵S]methionine/cysteine. Labeled proteins were resolved by electrophoresis through a 10% SDS polyacrylamide gel (top panel). To monitor the efficacy of tunicamycin treatment, endogenous CPY was immunoprecipitated from lysates of each time point and resolved by SDS-PAGE (bottom panel). An asterisk denotes the position of nonglycosylated pro-CPY. The positions of ER pro-CPY (p1) and Golgi pro-CPY(p2) are indicated.