Figure 2.

cdc31p localizes to the SPB at all phases of the cell cycle. (A) Top: Triple staining of wild-type cells for cdc31p (red), MTs (green), and DNA (blue). Bar, 5 μm. Bottom: Series of similarly stained cells at different cell cycle stages. From left to right: early G2, early mitosis, early and late anaphase, and postmitotic cell. (B) Staining of a GFP-cam1 strain for cdc31p. cdc31p staining (left) and GFP fluorescence (right) after formaldehyde fixation and processing for immunofluorescence are shown. Arrows point at SPBs. Bar, 5 μm.