Figure 4.

The developmental phenotypes of PHG1 knockout cells reveal a synergistic effect of PHG1a and PHG1b mutations. Wild-type cells, PHG1a, PHG1b, and PHG1a/PHG1b knockout cells were deposited on filters at the indicated temperature and observed for development under a dissection microscope. PHG1a knockout cells were arrested at the finger stage at 20°C or 25°C. PHG1b knockout cells exhibited a normal development at 20°C but were arrested at the early culmination stage at 25°C. PHG1a/PHG1b double knockout cells were stopped at the early culmination stage at 20°c and at the tipped mound stage at 25°C.