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Effect of nocodazole and BFA on endogenous arfophilin-2 distribution. HeLa cells treated with or without (control) brefeldin A (20 μg/ml for 10 min) or nocodazole (50 ng/ml for 1 h) were fixed and stained with antibodies against arfophilin-2 (red in merged images), tubulin, TfR, or the CI-MPR (green in merged images). The experiment was repeated four times with similar results.
