Figure 8.

TfR traffic is not significantly perturbed in cells overexpressing GFP-arfophilin-2. HeLa cells expressing GFP-arfophilin-2 were incubated with ¹²⁵I-Tf for 2 h at 37C, washed in ice-cold citrate buffer at 4°C to remove cell surface-associated Tf, and then rewarmed into buffer at 37°C. The rate of Tf externalization was assayed by measurement of radioactivity associated with the cells at the times shown. The data shown are from triplicate determinations at each time point from a typical experiment, repeated three times with similar results.