Figure 7.

STX6 cytosolic domain perturbs Glut4 endosomal sorting. The LDM fractions from cells treated as described in the legend to Figure 6 were subjected to iodixanol gradient analysis. Fractions were collected from the bottom of the gradient (left of figure), and SDS-PAGE was performed using 10% of each fraction. The distribution of Glut4 was then determined by immunoblotting, and the position of the GSV and TGN/endosomal Glut4 peaks are indicated. The representative experiment shown was repeated twice with qualitatively similar data.