Table 2.

DNA synthesis in antisense-cdc25C injected cells following cdc25C protein microinjection.

Microinjection	No. of cells reinjected	Number of cell positive for BrdU	% cells positive for BrdU
Cdc25C-wt into antisense plasmid p25as cells	22	18	82
Cdc25C-wt into antisense oligo AS1 cells	49	30	62
Cdc25C-C377S into antisense oligo AS1 cells	42	3	7

Background DNA synthesis levels was 96%. Hs68 fibroblasts were synchronised by serum starvation. Five hours after serum readdition cells were microinjected with p25Cas or oligonucleotide AS1 and Lucifer Yellow (LY). Twenty-two hours after refeeding, injected cells were identified by the presence of LY in the cytoplasm and reinjected with active cdc25C-wt, inactive cdc25C-C377S or cyclin A. Immediately after the second injection cells were further cultured in the presence of BrdU for 4 h. Cells were then fixed and stained for rabbit marker antibodies (for the number of cells reinjected) and BrdU incorporation (for cells that have entered S-phase). Total results from two independent experiments are shown. DNA synthesis in antisense-injected, but not re-injected, surrounding cells was 14% (for p25as) and 15% (for AS1)