Table 3.

DNA synthesis in cdc25C siRNA-transfected cells following cdc25C protein microinjection.

Microinjection	No. of cells injected	Number of cell positive for BrdU	% of cells rescued
Cdc25C-wt	84	76	90
Cdc25C-C377S	77	5	6
Marker antibodies	90	5	5

Background level of DNA synthesis was 5%. Asynchronously growing HeLa cells were transfected with siRNA duplexes targeting cdc25C. Thirty-six hours after transfection, cells were microinjected with active cdc25C-wt protein, inactive C377S mutant protein, or microinjection marker antibodies alone. Cells were further cultured in the presence of BrdU for 4 h before fixation. Subsequently cells were co-stained for rabbit marker antibodies (for the number of cells injected) and BrdU (for the number of cells that have entered S-phase). Numbers represent mean values from four independent experiments