Figure 9.

Distribution of rootlet microtubules in wild-type and mutant strains. (A) In wild-type cells, four bundles of rootlet microtubules form a cruciate array. The triplet microtubules of one basal body (green) were modeled for reference. Four-membered rootlets display a 3-over-1 microtubule arrangement (pink) and two membered rootlets contain two roughly parallel microtubles. (B) Examples of rootlet microtubule bundles from two different uni3-1 cells. The basal bodies are formed from mostly doublet microtubules (green) although some triplets can be detected. The rootlet microtuble bundles look disorganized. Some four-membered bundles (pink) are present, but often they do not form a 3-over-1 arrangement. Two–membered bundles were observed (blue) as well as microbules that did not bundle (yellow). (C) tua2-6; uni3-1 cells contained both four-membered (pink) and two membered (blue) but their arrangement was not completely normal. The basal body (green) contains both doublet and triplet microtubles. (D) Cells that are simply tua2-6 contain basal bodies with triplet microtubules (green) and four-membered (pink) and two-membered (blue) rootlet microtubule bundles with a normal, cruciate arrangement.