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. 2006 Nov 21;103(49):18745–18750. doi: 10.1073/pnas.0609012103

Fig. 3.

Fig. 3.

The absence of SdhA causes death of infected macrophages. (A) Trafficking of L pneumophila sdhA::Tn10 in macrophages. Bone marrow macrophages were incubated with noted strains, and percent colocalization with the late endosomal marker LAMP-1 was determined by immunofluorescence. Strains used were as follows: LP02 (Dot+); LP03 (dotA−); and RL1010 (sdhA−). (B and C) Loss of macrophages infected by strain lacking SdhA protein. Bone marrow-derived macrophages were incubated with strains for denoted lengths of time, and the number of infected macrophages was determined. (D–F) Bone marrow macrophages incubated with L. pneumophila sdhA::Tn10 are TUNEL-reactive. Macrophages were incubated with L. pneumophila strains for 10 h, stained for bacteria and TUNEL reaction, and observed microscopically. Infected cells, macrophages harboring bacteria. Uninfected cells, macrophages that have no observable associated bacteria. (G) Increased caspase activation in macrophages harboring L. pneumophila ΔsdhA. Macrophages were incubated for 1 or 8 h with L. pneumophila strains, and cells were stained with FITC-VAD-FMK.