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. 2006 Nov 21;103(49):18745–18750. doi: 10.1073/pnas.0609012103

Fig. 5.

Fig. 5.

Inhibition of caspase activity does not fully rescue the intracellular growth defect of a L. pneumophila ΔsdhA strain. (A) Enhanced intracellular replication of a L. pneumophila ΔsdhA mutant. Macrophages were incubated in the presence of Z-VAD-FMK throughout the incubation period. (B) Cells with aberrant morphologies in the presence of Z-VAD-FMK have multiple TUNEL phenotypes. Arrows point to cells with aberrant morphologies. (C) zVAD-FMK is unable to protect against mitochondrial disruption by 8 h postinfection. Displayed are macrophages showing punctate cytochrome c staining in the presence of z-VAD-FMK. (D) Protection by caspase inhibitor is transient. Noted strains were assayed for ethidium bromide (EtdBr) permeability at 1 h and 8 h postinfection in the presence or absence of Z-VAD-FMK, after incubation with L. pneumophila at moi = 1.0.