Fig. 4.

Characterization of hair-cell regeneration in several zebrafish mutant lines. (A–C) Gastrulation defects in zebrafish are associated with the silverblick/wnt11, pipetail/wnt5a, and knypek mutations (21–23). Labeling of neuromasts in 6-day-old larvae with rhodamine-phalloidin reveals that the hair bundles are properly orientated in pipetail (A), silberblick (B), and knypek mutant animals (C). (D) A neuromast of a trilobite mutant animal is included for comparison. The planar polarity defects of hair cells in trilobite mutants are associated with the loss of Vangl2 function specifically in neuromasts, rather than a consequence of gastrulation defects. (Scale bars: 10 μm.)