Fig. 2.
Isolation and characterization of AsCyp51H10. (A) BAC clone #B460D15 contains Sad1 and AsCyp51H10. (B) Gene structures of AsCyp51H10, AsCyp51H10, and AsCyp51G1. (C) Northern blot analysis of AsCyp51H10 and AsCyp51G1 transcripts (Left) and RT-PCR analysis of AsCyp51H11 and AsCyp51G1 transcripts (Right) in oat roots (R), shoots (S), leaves (L), and flowers (F). The oat glyceraldehyde 3-phosphate dehydrogenase gene (GAPDH) was used as a control for RT-PCR. (D) In situ mRNA analysis of Sad1 and AsCyp51H10 transcripts in the root tips of A. strigosa.