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Identification and characterization of sad2 mutants. (A) GC analysis of root extracts from S75 (WT) and sad1 and sad2 mutants. The vertical bar on the bottom left indicates relative mass abundance. sad2 mutants accumulate β-amyrin, whereas sad1 mutants (such as mutant #109) accumulate 2,3-oxidosqualene. The identity of the accumulated intermediates was determined by MS. (B) Northern blot analysis of AsCyp51H10 transcript levels in sad2 mutants and the WT S75.
