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(a) The mRNA expression of iNOS was assessed by using RT-PCR as standardized by coamplifying the housekeeping gene β-actin. Lanes 1–7: Marker, Vehicle, Sham, TF1 (5 mg · kg−1), TF1 (10 mg · kg−1), TF1 (20 mg · kg−1), Nimodipine. (b) Statistical analysis revealed that theaflavin-treatment markedly decreased mRNA expression of iNOS dose dependently, **P < .01.
