FIG. 7.

Effect of Y2^−/− Y4^−/− double knockout on CRH and TRH mRNA levels in neurons of the paraventricular nucleus (upper panels); on NPY, AgRP, POMC, and CART mRNA levels in the arcuate nucleus (middle panels); and on GnRH mRNA levels in forebrain neurons (lower panels) in male mice. High-power bright-field photomicrographs of dipped sections obtained from control and Y2^−/− Y4^−/− double knockout mice after in situ hybridization for the respective mRNA are shown. Bar, 100 μm. The paraventricular nucleus is depicted at about −0.8 mm and the arcuate nucleus is depicted at about −1.8 to −1.9 mm from Bregma as low-magnification film autoradiographs for TRH and CART.