TABLE 3.
Expression levels of neuropeptide mRNAs in hypothalamic and forebrain nuclei of Y receptor knockout mice
| mRNA | Expressiona in the following mice:
|
|||
|---|---|---|---|---|
| Control | Y2−/− | Y4−/− | Y2−/− Y4−/− | |
| Paraventricular nucleus | ||||
| CRH | 100 ± 3.5 (5) | 66 ± 9.4 (10)*** | 103 ± 5.1 (4) | 69 ± 2.9 (5)*** |
| TRH | 100 ± 2.6 (6) | 58 ± 5.5 (10)*** | 113 ± 6.9 (4) | 117 ± 5.9 (5)* |
| Arcuate nucleus | ||||
| NPY | 100 ± 4.2 (10) | 115 ± 7.5 (10)* | 100 ± 2.4 (10) | 131 ± 10 (5)** |
| AgRP | 100 ± 3.3 (9) | 126 ± 5.7 (10)** | 99 ± 6.7 (10) | 120 ± 1.8 (10)** |
| POMC | 100 ± 2.7 (10) | 88 ± 3.5 (10)* | 99 ± 2.2 (9) | 100 ± 4.5 (5) |
| CART | 100 ± 2.7 (10) | 79 ± 4.4 (10)** | 103 ± 2.4 (9) | 88 ± 6.7 (5)* |
| Forebrain neurons (GnRH) | 100 ± 6.6 (5) | 104 ± 0.9 (5) | 123 ± 8.5 (5)** | 119 ± 3.8 (5)* |
a
Data represent mean labeling intensity of neurons, given as percentage of the control value, ± standard error of the mean for the number of mice shown in parentheses. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (versus control).