Figure 1.

Transgene expression [β-galactosidase (β-gal)] and inflammatory responses (CD8, MHC I, ED1) in the brain, 21 days after intradermal injection of adenovirus. Rows a–d show brains from animals injected intrastriatally with 1.3 × 10⁷ i.u. of RAd35 (rows a and b) or AdGS46 (rows c and d) at day 0, and intradermally with 5 × 10⁸ i.u. of RAdHPRT (rows a and c), or the equivalent number of particles of psoralen-inactivated RAdHPRT (ps-RAdHPRT, rows b and d) at day 60. Bold font indicates the vector used for the striatal injection and normal font indicates the vector used for the dermal injection. Perfused-fixed brains from animals killed 21 days after the intradermal injection (i.e., day 81 post-brain injection) were cut into serial 50-μm sections and were stained for β-galactosidase (shown at low magnification in the first column with boxed area at high magnification in the second column), CD8⁺ cells (third column), MHC class I (fourth column), and activated microglial cells and macrophages (ED1 immunoreactivity; fifth column). Red arrowheads indicate the needle track. [Bar = 1 mm (in row d of the first column; scale for entire first column) and 200 μm (in row d of the fifth column; scale for the second through fifth columns).]