Figure 5.

Immunohistochemical detection of transgene expression and inflammatory responses in the brain, 3 and 30 days after intrastriatal injection of a single dose of adenovirus. Rows a (3 days postinfection) and b (30 days postinfection) show the immunohistochemical detection of transgene expression and inflammatory markers, after a single administration of the first-generation vector RAd35, or the high-capacity vector AdGS46 to the brains of naïve animals (n = 3 per virus, per time point). Adenovirus-induced brain infiltration of CD8⁺ cells, ED1⁺ macrophages, and up-regulation of MHC class I expression subsided within 30 days whereas levels of β-galactosidase expression remained stable. Red arrowheads indicate the needle track. [Bar = 1 mm (in row b of the first column; scale for the low magnification images) and 150 μm (in row b of the fifth column; scale for the higher magnification images).]