TABLE 2.
Kinetic characteristics of RIC-mediated import
| Experimental variable | Parameter | Value |
|---|---|---|
| tRNATyr | Kma | 1.16 nM |
| ATP | Kma | 1.35 mM |
| tRNATyr (effector) on tRNAIle (substrate) | EC50b | 0.033 nM |
| tRNAIle (effector) on tRNATyr (substrate) | IC50c | 0.018 nM |
a
Concentration of tRNATyr or ATP for half-maximal rate of import into RIC-reconstituted phosphatidylcholine vesicles.
b
Concentration of tRNATyr effector required for half-maximal stimulation of binding of tRNAIle (2 nM).
c
Concentration of tRNAIle effector for half-maximal inhibition of binding of tRNATyr (2 nM).