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. 2003 Aug;23(15):5198–5207. doi: 10.1128/MCB.23.15.5198-5207.2003

FIG. 3.

FIG. 3.

Syntaxin 8 protein levels are reduced in vti1b-deficient mice. Homogenates were prepared from livers, brains, kidneys, and spleens of wild-type (+/+), heterozygous (+/−), and vti1b-deficient (−/−) animals; separated by SDS-PAGE; and stained with antisera directed against the indicated SNAREs. Liver homogenates from wild-type and vti1b-deficient animals were extracted with Triton X-114, and the detergent phase was separated by SDS-PAGE to allow the detection of endobrevin. No reduction in protein levels was observed for endobrevin, syntaxin 7, vti1a, and SNAP-29.