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. 2003 Aug;23(15):5132–5142. doi: 10.1128/MCB.23.15.5132-5142.2003

FIG. 2.

FIG. 2.

The sws1 mutant is defective in the expression of the stress-response genes regulated by Atf1. (A) Wild-type (PR109) and sws1-681 (CA1388) strains were grown to the mid-log phase at 30°C in YES medium and treated with oxidative stress induced by 0.3 mM H2O2. Aliquots of cells were taken at the indicated times, and total RNA was extracted for the Northern blot analysis of gpd1+ and pyp2+ mRNA. The leu1 probe served as a loading control. (B) The levels of pyp2+ mRNA in the experiment shown in panel A were quantified and normalized by the leu1 mRNA levels using the Storm system (Molecular Dynamics). Numbers are in arbitrary units.