TABLE 1.

Intracellular localization of wild-type and mutant RAG-2 proteins expressed in the absence of RAG-1 in NIH 3T3 cells^a

Protein, segment, or mutation	Localization (% of cells)
	N	N > C	N = C	C
GFP	0	11	89	0
RAG-2 (wild-type)	73	25	2	0
1-517	0	80	19	1
1-498	0	7	93	0
1-387	1	0	96	3
439-527	81	14	5	0
439/448A10	14	70	15	2
449/458A10	32	58	8	2
459/468A10	62	22	16	0
469/478A10	31	57	11	1
479/488A10	35	56	8	1
489/498A10	0	16	84	0
499/508A10	0	4	93	2
509/518A10	0	7	83	9
519/528A10	0	0	27	73
489/508A10	0	1	95	4
K499A	55	43	2	0
M502A	0	51	38	11
K503A	0	19	80	2
KK507/508A2	0	20	68	13
K499A; K503A; KK507/508A2	0	23	73	4
KPPM499/502A4; SLH504/506A3	0	29	62	10
K499A; K503R; KK507/508R2	0	38	54	8
T490A	84	15	1	0
K492A	40	51	9	0
R493A	21	72	7	0
KR492/493A2	18	66	16	0
NPPLQ494/498A5	0	63	30	7
KRNNPLQ492/498A7	0	5	93	2
K512A	9	74	17	0
K518A	29	53	16	2
K519A	19	65	13	2
KK518/519A2	0	59	34	7
R523A	1	79	20	0
R524A	50	43	7	0
RR523/524A2	0	56	43	1
KR492/493A2; K512A	0	37	39	25
KR492/493A2; K523A	0	19	81	0
LacZ	0	0	18	82
LacZ-RAG-2(439-527)	67	29	4	0

^aN, nuclear; N > C, more nuclear than cytoplasmic; N = C, diffuse in the cell; C, cytoplasmic.