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. 2003 Aug;23(15):5165–5173. doi: 10.1128/MCB.23.15.5165-5173.2003

FIG. 4.

FIG. 4.

Ectopic expression of SKP2 does not affect the expression of CDK9. (A) T98G cells were serum starved and restimulated (left panels); serum starved, infected with recombinant Ad expressing SKP2 (Ad-SKP2), and restimulated (middle panels); or serum starved and infected with Ad-SKP2 (right panels). Cells were collected at the indicated time points after restimulation or infection, and the expression levels of SKP2, CDK9, and p27 proteins were analyzed by Western blotting. SS, serum starved. (B) Exponentially growing HeLa cells were infected with the indicated recombinant Ad. The cells were collected 48 h after infection, and the expression levels of SKP2, CDK9, and p27 proteins were analyzed by Western blotting. (C) Effect of the ectopic expression of cyclin T1 on CDK9 stability. HeLa cells were infected with combinations of recombinant Ad expressing CDK9-HA (Ad-CDK9; constant MOI) and recombinant Ad expressing cyclin T1-HA (Ad-cyclin T1; increasing MOI). Forty-eight h postinfection, the cells were collected and the expression levels of cyclin T1 and CDK9 proteins were analyzed by Western blotting in the cell lysates.