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. 2000 Jun 13;97(13):7488–7493. doi: 10.1073/pnas.120184097

Figure 3.

Figure 3

A multiple-tissue cDNA panel (MTC, CLONTECH), containing normalized first-strand cDNA (Left to Right) brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, positive control (combined tissues), no DNA control, and GPDH control, was PCR amplified with apoB48R sequence-specific primers overlapping introns 2 and 3 (see supplemental Table 1) to detect only mRNA. The expected 455-bp product was found in all but skeletal muscle, with most in lung and placenta (seen after 25 cycles) and least in brain and heart (seen after 35 cycles). Control PCR analyses for the GPDH cDNA verified normalization of mRNA levels.