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(A) ApoB48R-transfected CHO were incubated at 37° for 3 h with CM Sf >400, with apoB48 as the only apoB species, at 100 μg TG/ml RPMI and stained with Oil red O to detect cytoplasmic neutral lipid droplets (2). (B) Vector only-transfected CHOs were incubated with CM under identical conditions and stained with Oil red O. R- and vector-transfected cells treated with buffer had no lipid staining inclusions (not shown).
