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. 2003 Aug;23(15):5143–5164. doi: 10.1128/MCB.23.15.5143-5164.2003

FIG. 6.

FIG. 6.

EMSA analysis of in vitro-transcribed and translated NTEF-1, RTEF-1a, and RTEF-1b proteins. 32P-labeled βA/T-rich element was reacted with 2 μl of in vitro-synthesized NTEF-1 (A, lane 2), RTEF-1a (B, lane 14), and RTEF-1b (C, lane 26). For competition assays, the following nonradioactive oligonucleotides were added to the binding reactions at 100-fold molar excess prior to the addition of the 32P-labeled βA/T-rich probe: βA/T-rich wild type (lanes 3, 15, and 27); βA/T-rich mut (lanes 4, 16, and 28); α-MyHC GATA (lanes 5, 17, and 29); cTnC GATA (lanes 6, 18, and 30); BNP GATA (lanes 7, 19, and 31); desmin MEF2 (lanes 8, 20, and 32); myoglobin MEF2 (lanes 9, 21, and 33); MCK Trex (lanes 10, 22, and 34); βMyHC dMCAT (lanes 11, 23, and 35); free probe (lanes 12, 24, and 36). Notably, the addition of the desmin MEF2 element to binding reactions containing NTEF-1 (lane 8), RTEF-1a (lane 20), and RTEF-1b (lane 32) resulted in an unexpected inhibition of complex formation.