TABLE 3.
Effect of eIF1 and eIF5 overexpression on the mild Sui− phenotype of tif4632-1
| Transformanta | Host strain | Plasmid 1b | Plasmid 2 (his4::lacZ)c | Units of β-galactosidased | UUG/AUG ratioe |
|---|---|---|---|---|---|
| 1 | YAS1951 (WT) | Vector | p367 (AUG) | 431 ± 64 | 3.8 (1) |
| 2 | YAS1951 (WT) | Vector | p391 (UUG) | 16 ± 4 | |
| 3 | YAS1951 (WT) | High-copy-number eIF1 | p367 (AUG) | 468 ± 50 | 3.5 (0.93) |
| 4 | YAS1951 (WT) | High-copy-number eIF1 | p391 (UUG) | 16 ± 6 | |
| 5 | YAS1951 (WT) | High-copy-number eIF5 | p367 (AUG) | 665 ± 77 | 3.6 (0.96) |
| 6 | YAS1951 (WT) | High-copy-number eIF5 | p391 (UUG) | 24 ± 4 | |
| 7 | YAS1998 (tif4632-1) | Vector | p367 (AUG) | 443 ± 95 | 12.3 (3.2) |
| 8 | YAS1998 (tif4632-1) | Vector | p391 (UUG) | 54 ± 14 | |
| 9 | YAS1998 (tif4632-1) | High-copy-number eIF1 | p367 (AUG) | 957 ± 221 | 6.4 (1.7) |
| 10 | YAS1998 (tif4632-1) | High-copy-number eIF1 | p391 (UUG) | 61 ± 11 | |
| 11 | YAS1998 (tif4632-1) | High-copy-number eIF5 | p367 (AUG) | 582 ± 149 | 10.9 (2.9) |
| 12 | YAS1998 (tif4632-1) | High-copy-number eIF5 | p391 (UUG) | 63 ± 10 |
a
Transformants of indicated host strain carrying plasmids 1 and 2 were grown in SC-ura-ade medium and assayed exactly as described in the legend to Fig. 7.
b
Either pRS422 (ADE2 2μm; Vector) or a derivative of pRS422 carrying SUI1 or TIF5 (high-copy-number eIF1 or eIF5, respectively).
c
Either p367 or p391 as listed in Table 1.
d
Defined in the legend to Fig. 7.
e
Ratio (percent) of expression from p391 (UUG) over that from p367 (AUG). Values in parentheses are the UUG/AUG ratio relative to that for YAS1951/pRS422, which was used to judge a Sui− mutant phenotype.