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Rgt1 binds with Ssn6-Tup1 on an HXT promoter. Chromatin prepared from yeast cells (WT, YM4127; Δssn6, YM4554; Δrgt1, YM4509) grown on YP containing different carbon sources as indicated above each lane was immunoprecipitated using anti-Ssn6 antibody (Santa Cruz). The HXT3 promoter in the immunoprecipitated DNA (IP) was amplified in a PCR, resolved in an agarose gel (2%), and visualized by ethidium bromide staining.
