FIG. 7.

Glucose does not regulate Rgt1 levels or nuclear localization. (A) Yeast cells (YM4509) expressing LexA-Rgt1 (expressed from pBM3307) were grown on minimal medium containing either 2% galactose or 2% raffinose (equivalent to low levels of glucose) or 4% glucose. Yeast cells (YM4509) expressing only LexA (pBM2662) were grown on minimal medium containing 2% galactose (Vector). LexA-Rgt1 was immunoprecipitated and subjected to Western blot analysis using anti-LexA antibody as a probe. (B) Yeast cells (YM4127) expressing GFP-Rgt1 (expressed from pBM3911) were grown to mid-log phase under repressing conditions (2% galactose), the carbon source was switched to 4% glucose, and growth was continued for 1.5 h. The cells were harvested and stained with DAPI before and after the carbon source was switched and then were imaged for DAPI and GFP fluorescence or by Nomarski optics.