FIG. 3.

Expression of Myc-hFis1 or untagged hFis1 induces fragmentation of mitochondria. Clone 9 cells carrying GFP-labeled mitochondria (GFP-mito) were transfected with Myc-hFis1 and subjected to indirect immunofluorescence with antibodies to Myc. Cells expressing Myc-hFis1 (A) (asterisk) contained completely fragmented mitochondria (A′) to which Myc-hFis1 localized (arrows). Note the normal mitochondria in the untransfected cell in panel A′ (Un). (B) Clone 9 cells carrying RFP-labeled mitochondria (RFP-mito) were microinjected with untagged, full-length hFis1 DNA (UT-hFis1) along with GFP vector as a transfection marker. Mitochondria became fragmented in cells expressing untagged hFis1. Bar, 10 μm.