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. 2003 Aug;23(15):5409–5420. doi: 10.1128/MCB.23.15.5409-5420.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 6. — Disruption of hFis1 function alters mitochondrial shape and distribution. (A and A′) Clone 9 cells harboring GFP-mitochondria were transfected with Myc-hFis1ΔC. Mitochondria in the cell overexpressing Myc-hFis1ΔC (arrows) displayed a collapsed phenotype. (B and B′) Anti-hFis1 antibodies were microinjected into Clone 9 cells harboring GFP-mitochondria to inhibit hFis1 function. By 2 h, mitochondria in antibody-injected cells (arrows) became elongated and collapsed around the nucleus. (C and C′) Antisense oligonucleotides were introduced into Clone 9 cells to reduce the expression of hFis1. At 48 h posttransfection, cells were either fixed for immunofluorescence or lysed for immunoblotting. A 50% reduction of hFis1 expression was observed when normalized to tubulin by immunoblotting (inset). Anti-cytochrome c staining showed that cells (arrows) displayed a fission-defective mitochondrial morphology. N, nucleus; S, sense; AS, antisense.