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Exo1 and rad50 function independently upstream of Rad51. (A) Indirect immunofluorescence microscopy of wild-type cells (700), rad50-d (701), exo1-d (702), and rad50-d exo1-d cells (703) at 1 h after irradiation with 500 Gy of ionizing radiation (8). A cross-reacting human anti-Rad51 antibody was used to detect Rhp51. (B) Mean percentage of various mutant cells showing Rhp51 foci at 1 h postirradiation.
