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. 2000 Jun 20;97(13):7494–7499. doi: 10.1073/pnas.97.13.7494

Figure 2.

Figure 2

Activation of caspase-3 by BBL22. (A) Immunoblot analysis of caspase-3 in LNCaP cells before (lane 1) and 72 h after (lane 2) treatment with 50 μM BBL22. The 32-kDa procaspase-3 form (arrow) is the predominant species in untreated cells. After treatment with BBL22, procaspase-3 is cleaved to the active 17-kDa product (arrowhead). (B) Proteolytic cleavage of procaspase-3 (arrow) to the active caspase-3 form (arrowhead) after treatment of HBL100 cells with BBL22 (as described previously). Untreated controls (lane 1), 25 μM BBL22 for 72 h (lane 2), and 50 μM BBL22 for 72 h (lane 3). Equal amounts of total cell lysate were loaded to each lane in both A and B.