Table 2.

BBL22 cell cycle arrest is cumulative and not abrogated by saturation of PBR binding sites

Culture conditions in MCF7 cells	% G1	% S phase	% G2/M
Control	68	12	10
BBL22, 24 h	46	10	40
BBL22, 24 h followed by 72-h washout period*	20	10	70
Saturation of PBR (preincubation with 50 μM Ro5-4864) before BBL22, 24 h	43	16	38

These results are representative of four independent experiments. 

^*Cells were cocultured with 50 μM BBL22 for 24 h, then replaced in drug-free medium, and cell cycle analysis was performed 72 h later.