Table 3.

Structural requirements for antitumor effects of peripheral-acting BZD

Culture condition	% Sub-G1	% G1	% S phase	% G2/M
Control	<1	70	14	16
Ro5-4864	<1	68	17	13
PK11195	<1	69	13	19

PC3 cells in exponential log growth phase were cocultured with vehicle alone (control), Ro5-4864 (50 μM), or PK11195 (50 μM) for 24 h. Subsequent cell cycle analysis at 72 h revealed no further effect of either compound on cell cycle kinetics. These results are representative of five independent experiments. No effect on cell cycle was observed when tumor cells were cocultured with the following peripheral-acting BZD compounds (50 μM for 72 h): Ro22-3793, Ro5-6900, Ro5-5120, Ro11-6896, Ro5-5115, Ro7-3371, Ro5-3463, Ro7-5220, and diazepam.