FIG. 3.

Cdc34 self-association is not dependent upon a functional SCF complex. Flag-Cdc34 and Myc-Cdc34 were expressed in 15DaubΔ (wild type [wt]) or DSY1105 (cdc53-1) cells at either the permissive (25°C) or nonpermissive (37°C) temperature. Cell extracts were prepared, and Myc-Cdc34 was immunoprecipitated (IP) with an anti-Myc antibody, followed by immunoblotting (IB) with either an anti-Myc antibody (right panel) or an anti-Flag antibody (left panel). The asterisk indicates a proteolytic product of Cdc34. Protein expression levels within the lysates used for immunoprecipitations are shown at the bottoms of the respective panels.