FIG. 9.

Determinants of Cdc34 self-association. The crystal structure of the S. cerevisiae Ubc7 catalytic domain (6) is used here as a template for highlighting key residues within Cdc34 and is represented as both a ribbon diagram (top) and a surface model (bottom). The amino terminus is found at the top of the structures and the carboxy terminus is found at the bottom. The residue that corresponds to residue 170, where the carboxy-terminal extension of Cdc34 would start, is highlighted (orange). Amino acid residues that play a role in Cdc34 self-association are also highlighted and include the active-site residue C95 (yellow), S73 (purple), S97 (light blue), and the catalytic domain insert (amino acids 103 to 114) (red).