FIG. 7.

Tax induces a chromosome segregation defect in S. cerevisiae and human cells. (A) AFS173 cells containing Gal 10-Tax or Gal10-ΔTax were induced for LacI-GFP and Tax or ΔTax expression as described in Materials and Methods. Fluorescence microscopy was carried out with an Olympus IX70 microscope. WT-Tax, Tax⁺ cells containing two or more fluorescent spots are marked with arrows. Inset: a Tax⁺ cell with at least three fluorescent spots. ΔTax, Tax-null cells with single fluorescence spots. (B) Metaphase chromosome spread of human diploid fibroblast WI-38 cells infected with a high-titered stock of the pBabe-tax or pBabe-puro (pBabe) retrovirus vector.