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Subnuclear focus formation of Rad51 in response to MMC treatment. (A to D) Wild-type (A and B) and fancg (C and D) cells were analyzed 5 h after treatment with MMC (500 ng/ml for 1 h) (B and D) or were untreated (A and C). (E) Kinetics of Rad51 focus formation after the same MMC exposure. Cells with more than four distinct and bright foci were counted as positive. WT, wild type.
