TABLE 2.

Targeted integration frequency in wild-type and fancg mutant cells

Targeting construct	Targeted integration frequencya
	Wild type	fancg			Complemented with GdFANCG
		Clone 1	Clone 2	Clone 3
KU70-His	14/34 (41) [2.1 × 10−6]	10/46 (22) [2.0 × 10−6]	NDb	ND	22/36 (61) [3.8 × 10−6]
XRCC2-Bsr	16/37 (43) [2.0 × 10−6]	ND	6/24 (25) [7.9 × 10−6]	6/24 (25) [3.8 × 10−6]	18/28 (64) [4.8 × 10−6]
XRCC3-His	8/25 (32) [8.3 × 10−7]	1/22 (4.5) [7.3 × 10−7]	ND	ND	9/20 (45) [6.6 × 10−7]
Ovalbumin-Bsr	24/26 (92) [3.9 × 10−5]	24/28 (86) [3.8 × 10−5]	ND	ND	ND

^aData are numbers of targeted clones at each locus per number of drug resistant clones analyzed by Southern blotting. The percentage of targeted integration events is given in parentheses. Numbers in brackets indicate transfection efficiency. Results for three different fancg mutant clones and one clone complemented with chicken FANCG cDNA are shown.

^bND, not determined.