FIG. 4.

Binding of MinD mutants to the membrane. Binding of the MinD mutant proteins to the membrane was determined by a sedimentation assay. Either MinD (4 μM) (A) or the mutant proteins (B) were mixed with phospholipid vesicles (400 μg/ml) and ADP or ATP (1 mM). After a 20-min incubation at room temperature, the samples were centrifuged and the pellets were solubilized in SDS-sample buffer. Equivalent aliquots of the pellets and supernatants were analyzed by SDS-PAGE. WT, wild type; WD, double mutant L264W F268D.