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. 2003 Aug;185(15):4326–4335. doi: 10.1128/JB.185.15.4326-4335.2003

FIG. 9.

FIG. 9.

Effect of phospholipid vesicles and nucleotides on the fluorescence spectra of MinD mutant L267W. MinD mutant L267W (4 μM) was incubated with ADP or ATP in the absence (A) or presence (B) of phospholipid vesicles (100 μg/ml) and examined by fluorescence spectroscopy at 30°C. The excitation wavelength was 290 nm. (C) Effect of incorporating a spin label into the phospholipid vesicles on the emission spectra of MinD mutant L267W. MinD mutant L267W was incubated in the presence of phospholipid vesicles containing 10% doxyl-PC with the spin label at the 7 position of the sn-2 acyl chain. The asterisk indicates the presence of the spin label. a.u., arbitrary units.