TABLE 1.
Effect of minD mutations on MinD localization and MinC activation
| MinD in GFP-MinD fusion | Localization | Ability to activate MinCa [in strain JS964(pGB2minC) (Δmin)] on plates with:
|
|||
|---|---|---|---|---|---|
| Glucose | 0% Ara | 0.0001% Ara | 0.001% Ara | ||
| K260A K261A | Membrane | + | + | + | + |
| K260E | Membrane | + | + | + | + |
| K261E | Membrane | + | + | + | + |
| L264E L267E | Cytoplasm | − | − | − | +/− |
| K265A R266A | Membrane | + | + | + | + |
| F263E | Cytoplasm | − | − | +/− | + |
| F263W | Membrane | + | + | + | + |
| L264E | Cytoplasm | − | + | + | + |
| L264W | Membrane | + | + | + | + |
| L267E | Cytoplasm | − | + | + | + |
| L267W | Membrane | + | + | + | + |
| F268D | Cytoplasm | − | − | − | − |
| F268W | Membrane | + | + | + | + |
| L264W F268D | Cytoplasm | − | +/− | +/− | + |
| Wild type | Membrane | + | + | + | + |
| K16Q | Cytoplasm | − | − | − | − |
a
Activation was indicated by cell morphology. +, filamentation; +/−, slight filamentation; −, minicell phenotype with no filamentation. Ara, arabinose.