TABLE 3.
Ribosomal proteins of H. influenzae strain Rd KW20 identified in this study
| Protein | Genome identification no. | Size (amino acids) | Microaerobic conditionsa
|
Anaerobic conditionsa
|
Detection by 2DEc | ||||
|---|---|---|---|---|---|---|---|---|---|
| No. of peptides | No. of unique peptides | Identificationb | No. of peptides | No. of unique peptides | Identificationb | ||||
| RpS1 | HI1220 | 550 | 14 | 5 | + | 17 | 10 | + | − |
| RpS2 | HI0913 | 252 | 19 | 4 | + | 12 | 3 | + | <0.1 |
| RpS3 | HI0783 | 236 | 19 | 5 | + | 13 | 7 | + | <0.1 |
| RpS4 | HI0801 | 207 | 4 | 1 | + | 10 | 4 | + | + |
| RpS5 | HI0795 | 167 | 11 | 2 | + | 4 | 2 | + | + |
| RpS6 | HI0547 | 131 | 10 | 4 | + | 2 | 2 | + | − |
| RpS7 | HI0580 | 157 | 33 | 4 | + | 11 | 4 | + | − |
| RpS8 | HI0792 | 131 | − | 3 | 2 | + | + | ||
| RpS9 | HI1442 | 131 | − | − | + | ||||
| RpS10 | HI0776 | 119 | 9 | 1 | + | 16 | 13 | + | + |
| RpS11 | HI0800 | 130 | − | − | − | ||||
| RpS12 | HI0581 | 125 | − | − | − | ||||
| RpS13 | HI0799 | 123 | 8 | 2 | + | − | − | ||
| RpS14 | HI0791 | 102 | − | 2 | 1 | + | − | ||
| RpS15 | HI1468 | 90 | − | − | − | ||||
| RpS16 | HI0204 | 83 | 5 | 2 | + | 2 | 1 | + | − |
| RpS17 | HI0786 | 86 | 3 | 2 | + | 7 | 1 | + | − |
| RpS18 | HI0545 | 76 | − | 2 | 2 | + | − | ||
| RpS19 | HI0781 | 92 | 22 | 3 | + | 11 | 4 | + | − |
| RpS20 | HI0965 | 90 | 13 | 2 | + | 1 | 1 | ± | − |
| RpS21 | HI0531 | 72 | − | − | − | ||||
| RpL1 | HI0516 | 230 | 45 | 3 | + | 32 | 8 | + | − |
| RpL2 | HI0780 | 274 | 10 | 1 | + | 14 | 5 | + | <0.1 |
| RpL3 | HI0777 | 209 | 27 | 4 | + | 11 | 4 | + | + |
| RpL4 | HI0778 | 201 | 2 | 2 | + | 19 | 5 | + | + |
| RpL5 | HI0790 | 180 | 10 | 4 | + | 12 | 6 | + | + |
| RpL6 | HI0793 | 178 | 8 | 3 | + | 22 | 6 | + | + |
| RpL7/1 | HI0641 | 124 | 316 | 19 | + | 96 | 16 | + | − |
| RpL9 | HI0544 | 150 | 16 | 4 | + | 22 | 5 | + | − |
| RpL10 | HI0640 | 164 | 41 | 3 | + | 11 | 4 | + | − |
| RpL11 | HI0517 | 143 | 8 | 2 | + | 21 | 7 | + | − |
| RpL13 | HI1443 | 143 | 10 | 2 | + | 13 | 3 | + | + |
| RpL14 | HI0788 | 124 | − | 7 | 3 | + | − | ||
| RpL15 | HI0797 | 145 | 3 | 2 | + | 21 | 4 | + | − |
| RpL16 | HI0784 | 137 | 11 | 2 | + | 1 | 1 | + | − |
| RpL17 | HI0803 | 129 | 21 | 6 | + | 7 | 3 | + | + |
| RpL18 | HI0794 | 118 | 8 | 2 | + | 1 | 1 | + | − |
| RpL19 | HI0201 | 117 | − | 3 | 2 | + | − | ||
| RpL20 | HI1320 | 118 | 6 | 2 | + | − | − | ||
| RpL21 | HI0880 | 104 | 17 | 3 | + | 6 | 1 | + | + |
| RpL22 | HI0782 | 110 | 22 | 3 | + | 11 | 4 | + | − |
| RpL23 | HI0779 | 100 | 5 | 2 | + | 1 | 1 | ± | + |
| RpL24 | HI0789 | 104 | 24 | 1 | + | 43 | 3 | + | + |
| RpL25 | HI1630 | 96 | 15 | 3 | + | 3 | 1 | + | + |
| RpL27 | HI0879 | 86 | − | − | − | ||||
| RpL28 | HI0951 | 79 | 1 | 1 | +/− | 5 | 1 | + | − |
| RpL29 | HI0785 | 64 | 70 | 3 | + | 14 | 5 | + | − |
| RpL30 | HI0796 | 60 | − | − | − | ||||
| RpL31 | HI0758 | 71 | 3 | 2 | + | − | − | ||
| RpL32 | HI0158 | 57 | − | − | 4 | 2 | + | − | |
| RpL33 | HI0950 | 57 | − | − | − | − | |||
| RpL34 | HI0998 | 45 | − | − | − | − | |||
| RpL35 | HI1319m | 66 | − | − | − | − | |||
| RpL36 | HI0798.1 | 38 | − | − | − | − | |||
a
Cells were grown under microaerobic or anaerobic conditions as described in Materials and Methods.
b
+, protein identified with high confidence (see Table 1); ±, candidate protein; −, protein not detected.
c
Data from reference 34. <0.1, protein that was found on the gel at a low level and comprises no more than 0.1% of the total cell protein.