TABLE 2.
Effects of nhaR and rcsB mutations on osmotic and CPZ-induced stress responses of osmCp1
| Strain (relevant genotype) | β-Galactosidase activity (Miller units)a
|
|||
|---|---|---|---|---|
| No addition | NaCl (0.3 M) | LiCl (0.3 M) | CPZ (0.1 mM) | |
| CLG772 | 18 ± 2 | 70 ± 9 | 77 ± 6 | 92 ± 10 |
| CLG805 (rcsB::tet) | 13 ± 2 | 68 ± 5 | 62 ± 4 | 21 ± 1 |
| CLG806 (nhaR1::kan) | 14 ± 1 | 14 ± 2 | 15 ± 1 | 140 ± 15 |
| CLG809 (rcsB::tet nhaR1::kan) | 5 ± 1 | 7 ± 1 | 5 ± 1 | 5 ± 1 |
a
Cultures of the strains were grown aerobically in Luria-Bertani broth without NaCl at 37°C. At an optical density at 600 nm of 0.1, NaCl, LiCl, or CPZ was added, and the β-galactosidase activity was assayed after 2 h. The values are the means of the results of two independent experiments for CLG809 and three independent experiments for the other strains.