FIG. 2.

SDS-PAGE of the purified ATP synthase of Bacillus sp. strain TA2.A1. Samples were resolved on 12% polyacrylamide gels by the method of Schägger et al. (38) and were stained with Coomassie brilliant blue. (A) Lane 1, broad-range molecular mass marker (Bio-Rad) (the molecular masses [in kilodaltons] are indicated on the left); lane 2, purified Bacillus sp. strain TA2.A1 F₁F_o-ATP synthase (30 μg) (the positions of the subunits are indicated on the right). (B) Solubilization of hydrophobic subunits a and c with chloroform-methanol (1:1).